Cell Culture Surface

Monitoring Cell Culture Media Degradation using Surface

Sparger and Surface Gas Transfer for Cell Culture Bioreactors

The impact of bioreactor and sparger scale-up on gas transfer properties was investigated using four geometrically similar bioreactors ranging in size from 15-L to 2500-L total volume with the same sintered metal frit (microporous) sparger design. The mass transfer coefficients for both oxygen and carbon dioxide were measured at each scale at various flow rates. Scale did not directly affect ga...

Surface creasing instability of soft polyacrylamide cell culture substrates.

Efforts to understand and engineer cell behavior in mechanically soft environments frequently employ two-dimensional cell culture substrates consisting of thin hydrogel layers with low elastic modulus supported on rigid substrates to facilitate culturing, imaging, and analysis. Here we characterize how an elastic creasing instability of the gel surface may occur for the most widely used soft ce...

Mesenchymal Stem Cell Purification from the Articular Cartilage Cell Culture

Objective Articular cartilage as an avascular skeletal tissue possesses limited capacity to heal. On the other hand, it is believed that the regeneration capacity of each tissue is largely related to its stem cell contents. Little is known about the presence of mesenchymal stem cells in articular cartilage tissue. This subject is investigated in the present study. Materials and Methods Artic...

The Upper Cell Surface: Its Inability to Support Active Cell Movement in Culture

A variety of epithelial cells and fibroblasts fail to move over one another's upper surfaces in culture, resulting in monolayering. The failure of seeded fibroblasts to adhere to and spread on epithelial cell surfaces suggests that monolayering in culture is due to the lack of adhesion of the upper cell surface, at least of epithelial cells. Seeded fibroblasts and postmitotic, rounded fibroblas...